Phospho Chk1 (Ser345) Rabbit Polyclonal Antibody
Western blot analysis of extracts from HeLa cells,using Phospho-Chk1-S345 Rabbit PAb. at1:20000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.
| Catalog Number: | ABP-0578 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic phosphorylated peptide around S345 of human Phospho-Chk1-S345 |
| Reactivity: | Human,Mouse |
| Applications: | Western Blot: 1:1000 - 1:5000 |
| Molecular Weight: | 56kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
Applications
Western Blot: 1:1000 - 1:5000
Immunogen
A synthetic phosphorylated peptide around S345 of human Phospho-Chk1-S345