Actin alpha-Smooth Muscle
IHC(P) of Rat Cardiac Muscle Tissue using Anti-alpha-Smooth Muscle Actin antibody
| Catalog Number: | MABX-80133 |
| Conjugate: | Unconjugated |
| Size: | 200 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG2a |
| Clone: | 1A4 |
| Immunogen: | N-terminal synthetic decapeptide ofα-smooth muscle actin. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blot - At 2μg/ml with the appropriate system to detect α-SMA in cells and tissues Immunohistochemistry(P) - At 0.1-0.5 μg/ml to detect α-SMA in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) - At 0.3-1.2 μg/ml to detect α-SMA in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user. |
| Purification: | Purified by the goat anti-mouse IgG affinity chromatography. |
| Form: | Liquid |
Applications
Western blot - At 2μg/ml with the appropriate system to detect α-SMA in cells and tissues Immunohistochemistry(P) - At 0.1-0.5 μg/ml to detect α-SMA in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) - At 0.3-1.2 μg/ml to detect α-SMA in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
Immunogen
N-terminal synthetic decapeptide ofα-smooth muscle actin.