Actin beta Mouse Monoclonal Antibody
Western blot analysis of extracts of HeLa cells, using β-Actin Mouse mAb at 1:2000- 1:640000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB-24008 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1,Kappa |
| Clone: | AC-015 |
| Immunogen: | Fusion protein of human β-Actin |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Western Blot: 1:2000 - 1:20000Immunofluorescence: 1:50 - 1:200Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 42kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | BRWS1; PS1TP5BP1 |
Applications
Western Blot: 1:2000 - 1:20000Immunofluorescence: 1:50 - 1:200Immunocytochemistry: 1:50 - 1:200
Immunogen
Fusion protein of human β-Actin