Western blot analysis of extracts of mouse liver, 
using AFP antibody at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG 
(H+L) at 1:10000 dilution. 
Lysates/proteins: 25ug per lane. 
Blocking buffer: 3% nonfat dry milk in TBST. 
Detection: ECL Enhanced Kit.    
Exposure time: 60s

Western blot analysis of extracts of mouse liver, using AFP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s

Catalog Number:AB-84139
Size:50 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human AFP.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitaton: 1:50 - 1:100
Molecular Weight:68kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:AFP Alpha-fetoprotein antibodyAlpha-1-fetoprotein antibody Alphafetoglobulin AFP antibody HPAFP.

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitaton: 1:50 - 1:100

Immunogen

Recombinant protein of human AFP.