AMBRA1

Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-83947
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human AMBRA1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 -1:100
Molecular Weight:143kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:AMBRA1; DCAF3; WDR94; autophagy and beclin 1 regulator 1

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 -1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human AMBRA1