ASM

Western blot analysis of extracts of THP-1 cells, using ASM antibody at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 30s.
| Catalog Number: | AB-J199 |
| Size: | 100ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant protein of human SMPD1 / ASM |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100 |
| Molecular Weight: | 60kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | SMPD1;ASM;ASMASE;NPD;SMPD1/ASM |
Applications
Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100
Immunogen
Recombinant protein of human SMPD1 / ASM
