ATR (phospho-S428) Rabbit Polyclonal Antibody

Western blot analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.
| Catalog Number: | ABP10098 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic phosphorylated peptide around S428 of human ATR. |
| Reactivity: | Hu,man, Mouse, Rat |
| Applications: | Western Blot: 1:100 - 1:500 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 300kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | FRP1; MEC1; SCKL; FCTCS; SCKL1 |
Applications
Western Blot: 1:100 - 1:500 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Immunogen
A synthetic phosphorylated peptide around S428 of human ATR.
