ATR (phospho-S428) Rabbit Polyclonal Antibody

Western blot analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T
cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.      
Exposure time: 90s.

Western blot analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:ABP10098
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic phosphorylated peptide around S428 of human ATR.
Reactivity:Hu,man, Mouse, Rat
Applications:Western Blot: 1:100 - 1:500 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:300kDa
Purification:Affinity purification
Form:Liquid
Synonyms:FRP1; MEC1; SCKL; FCTCS; SCKL1

Applications

Western Blot: 1:100 - 1:500 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR.