BAX Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using BAX antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using BAX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Catalog Number:AB-82329
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500-1:1000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:21 kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:BCL2L4

Applications

Western Blot: 1:500-1:1000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX