Western blot analysis of extracts of various cell lines, using Bax antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using Bax antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84314
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide of human Bax
Reactivity:Hu, Ms, Rt
Applications:WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Molecular Weight:21kDa
Purification:Aff, Pur.
Form:Liquid
Synonyms:BCL2L4;BAX

Applications

WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200

Immunogen

A synthetic peptide of human Bax