BCL-2

Western blot analysis of extracts of various cell lines,
 using Bcl-2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
 (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-83984
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 81-131 of human Bcl-2.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:27kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:Bcl-2;PPP1R50;BCL2

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 81-131 of human Bcl-2.