BCL-2 Mouse Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody ) at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico plus.
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 10s.

Catalog Number:MAB-94760
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1, Kappa
Clone:AMC0441
Immunogen:Recombinant protein of mouse Bcl-2.
Reactivity:Human, Mouse, Rat
Applications:Western Blot:    1:500 - 1:1000 Immunohistochemistry:   1:100 - 1:500 Immunofluorescence:   1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:26KDa
Purification:Affinity purification
Form:Liquid
Synonyms:Bcl-2;PPP1R50;BCL2

Applications

Western Blot:    1:500 - 1:1000 Immunohistochemistry:   1:100 - 1:500 Immunofluorescence:   1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

Recombinant protein of mouse Bcl-2.