BDNF (Mature Form)
Western blot analysis of extracts of various cell lines, using BDNF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit. Exposure time: 90s.
| Catalog Number: | AB-83595 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant protein of human BDNF |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50-100 Immunofluorescence |
| Molecular Weight: | 14 kDa (mature BDNF) ~ 28-37 kDa (precursor BDNF) |
| Purification: | Aff. Pur. |
| Form: | Liquid |
Applications
Western Blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50-100 Immunofluorescence
Immunogen
Recombinant protein of human BDNF