Cadherin-E

Western blot analysis of extracts of HCT116 cells, using CDH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.

Western blot analysis of extracts of HCT116 cells, using CDH1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.

Catalog Number:MAB-94628
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG
Clone:CN-2
Immunogen:Recombinant protein of human E-Cadherin.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Molecular Weight:120kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:Arc-1;CD324;CDHE;ECAD;LCAM;UVO;CDH1;E-Cadherin

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant protein of human E-Cadherin.