Cadherin-E

Western blot analysis of extracts of various cell lines, using CDH1 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.     
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using CDH1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Catalog Number:AB-83857
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human CDH1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100 Immunofluorescence: 1:100
Molecular Weight:125kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CDH1; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; cad herin-1

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100 Immunofluorescence: 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human CDH1