Calbindin D28K Chicken Polyclonal Antibody

Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] parvalbumin, [7] calretinin, [8] calbindin. Bands at ~25kDa in tissue lysates and ~30 kDa in protein solutions correspond to native
calbindin, the recombinant forms of calbindin, caretinin and parvalbumin being slightly larger due to the presence of a His tag and other vector derived sequence. The Calb antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] parvalbumin, [7] calretinin, [8] calbindin. Bands at ~25kDa in tissue lysates and ~30 kDa in protein solutions correspond to native calbindin, the recombinant forms of calbindin, caretinin and parvalbumin being slightly larger due to the presence of a His tag and other vector derived sequence. The Calb antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

Catalog Number:AB-83902
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Chicken
Isotype:IgY
Clone:POLY
Immunogen:Full-length recombinant human protein expressed in and purified from E. coli.
Reactivity:Hu Rt Ms,Cw, Pg
Applications:Western Blot: 1:5,000 Immunofluorescence: 1,000-1:5,000 Immunocytochemistry: 1:1,000-1:5,000 Immunohistochemistry: 1;10,000
Molecular Weight: 28kDa
Purification:Purified
Form:Liquid

Applications

Western Blot: 1:5,000 Immunofluorescence: 1,000-1:5,000 Immunocytochemistry: 1:1,000-1:5,000 Immunohistochemistry: 1;10,000

Immunogen

Full-length recombinant human protein expressed in and purified from E. coli.