Caspase 3 Rabbit Polyclonal antibody

Western blot analysis of extracts of Jurkat cells at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Pico Exposure time: 3s. AB83240 can detect both the full length and the cleaved form.

Western blot analysis of extracts of Jurkat cells at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Pico Exposure time: 3s. AB83240 can detect both the full length and the cleaved form.

Catalog Number:AB-83835
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human CASP3
Reactivity:Human , Mouse , Rat
Applications:Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:Casp3: 35kDa - cleaved Casp3: 17kDa
Purification:Affinity purification
Form:Liquid

Applications

Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100

Immunogen

Recombinant protein of human CASP3