Caspase 3 Rabbit Polyclonal antibody

Western blot analysis of extracts of Jurkat cells at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Pico Exposure time: 3s. AB83240 can detect both the full length and the cleaved form.
| Catalog Number: | AB-83835 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant protein of human CASP3 |
| Reactivity: | Human , Mouse , Rat |
| Applications: | Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100 |
| Molecular Weight: | Casp3: 35kDa - cleaved Casp3: 17kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
Applications
Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100
Immunogen
Recombinant protein of human CASP3
