Caspase 8

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-82858
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human Caspase-8.
Reactivity:Ms, Rt, Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistryt: 1:50 - 1:200 Immunofluorecence: 1:50 - 1:200
Molecular Weight:17kDa/44kDa/60kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ALPS2B;CAP4;Casp-8;FLICE;MACH;MCH5;Caspase 8;CASP8;Caspase-8;caspase-8

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistryt: 1:50 - 1:200 Immunofluorecence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human Caspase-8.