CD100/SEMA42

Western blot analysis of extracts of
293T cells, using SEMA4D antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)  at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus. 
Exposure time: 10s.

Western blot analysis of extracts of 293T cells, using SEMA4D antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-84214
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 753-862 of human SEMA4D.
Reactivity:Hu
Applications:Western Blot: 1:200 - 1:1000
Molecular Weight:150kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SEMA4D;C9orf164;CD100;M-sema-G;SEMAJ;coll-

Applications

Western Blot: 1:200 - 1:1000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 753-862 of human SEMA4D.