CD3 epsilon Rabbit Polyclonal Antibody

Western blot analysis of extracts of
various cell lines, using CD3E
Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus 
Exposure time: 5s

Western blot analysis of extracts of various cell lines, using CD3E Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 5s

Catalog Number:AB-81831
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:RThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:300 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:21kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CD3E;IMD18;T3E;TCRE;CD3e molecule

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:300 Immunoprecipitation: 1:50 - 1:100

Immunogen

RThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.