CD31 / PECAM1 Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CD31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.
| Catalog Number: | AB-83615 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 Immunofluorescence: 1:50-1:200 |
| Molecular Weight: | 130kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | PECAM1;CD31;CD31/EndoCAM;GPIIA;PECA1;PECAM-1;e ndoCAM |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 Immunofluorescence: 1:50-1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31