CD324 / E-cadherin Mouse Monoclonal Antibody
Western blot analysis of extracts of HCT116 cells, using CDH1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.
| Catalog Number: | MAB-93240 |
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1 |
| Clone: | 67A4 |
| Immunogen: | T-47D cells |
| Reactivity: | Human |
| Applications: | Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry(paraffin-embedded tissues), Flow Cytometry Working dilutions: Immunohistochemistry (frozen sections):4-8 μg/ml Flow cytometry:5-10 μg/ml |
| Purification: | Purified by protein-A affinity chromatography. |
| Form: | Liquid |
| Synonyms: | Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1 |
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry(paraffin-embedded tissues), Flow Cytometry Working dilutions: Immunohistochemistry (frozen sections):4-8 μg/ml Flow cytometry:5-10 μg/ml
Immunogen
T-47D cells