CDKN1B
Western blot analysis of extracts from normal (control) and CDKN1B/p27KIP1 knockout (KO) HeLa cells, using CDKN1B/p27KIP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | AB-82085 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198of human CDKN1B/p27KIP1 (NP_004055.1). |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemsitry: 1:50 - 1:200 Immunofluorescence: 1:20 - 1:50 |
| Molecular Weight: | 27kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1 |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemsitry: 1:50 - 1:200 Immunofluorescence: 1:20 - 1:50
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-198of human CDKN1B/p27KIP1 (NP_004055.1).