Choline Acetyltransferase (ChAT) Rabbit Polyclonal Antibody
Western blot analysis of various lysates, using CHAT Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.
| Catalog Number: | AB-84238 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot 1:1000 - 1:2000 Immunohistochemistry (paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence:: 1:50 - 1:200 Immunocytcoehmistry: 1:50 - 1:200 |
| Molecular Weight: | 71kDa |
| Purification: | Affinity Purified |
| Form: | Liquid |
| Synonyms: | CMS6; CMS1A; CMS1A2; CHOACTASE; CHAT |
Applications
Western Blot 1:1000 - 1:2000 Immunohistochemistry (paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence:: 1:50 - 1:200 Immunocytcoehmistry: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT.