Claudin 1 Rabbit Monoclonal Antibody
Western blot analysis of lysates from wild type (WT) and Claudin 1 knockdown (KD) Hep G2 cells using Claudin 1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL west Pico Plus. Exposure time: 1s.
| Catalog Number: | MAB25902 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC3289 |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:1000 - 1:5000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF-P 1:50 - 1:200 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 23kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | CLD1; SEMP1; ILVASC |
Applications
WB 1:1000 - 1:5000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF-P 1:50 - 1:200 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.