Collagen I / COL1A2 Rabbit Polyclonal Antibody
Western blot analysis of extracts of A375 cells, using Collagen I/COL1A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.
| Catalog Number: | AB-84752 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:25 - 1:200 Immunocytochemistry: 1:25 - 1:200 |
| Molecular Weight: | 129KDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | OI4; EDSCV; EDSARTH2 |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:25 - 1:200 Immunocytochemistry: 1:25 - 1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2.