COLLAGEN II / COL2A1 Rabbit Polyclonal Antibody
Western blot analysis of lysates from 1) k562 , 2) COLO205,3) MCF-7 cells, Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. Red） Actin β Monoclonal Antibody(5B7)
| Catalog Number: | AB-84753 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen II. AA range:101-150 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000 ELISA:1/20000. |
| Molecular Weight: | 140 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | COL2A1; Collagen alpha-1(II) chain; Alpha-1 type II collagen |
Applications
Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000 ELISA:1/20000.
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen II. AA range:101-150