Collagen Type I Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using COL1A1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 3min.
| Catalog Number: | AB-82138 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, identical to the related rat sequence, and different from the related human sequence by two amino acids. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 -1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunohistochemistry: 1:50-1:200 |
| Molecular Weight: | 140kDa/220kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | COL1A1; EDSC; OI1; OI2; OI3; OI4 |
Applications
Western Blot: 1:500 -1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunohistochemistry: 1:50-1:200
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, identical to the related rat sequence, and different from the related human sequence by two amino acids.