CREB 2 / ATF4

Western blot analysis of extracts of HeLa cells, using ATF4 antibody   at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  
Exposure time: 3min.

Western blot analysis of extracts of HeLa cells, using ATF4 antibody at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:AB-82156
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Molecular Weight:50kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ATF4;CREB-2;CREB2;TAXREB67;TXREB

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4