CXCL10 Rabbit Polyclonal Antibody
Western blot analysis of THP-1, using CXCL10/IP-10 antibody at 1:3000 dilution.THP-1 cells were treated by LPS for 6 hours,Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.Exposure time: 30s.
| Catalog Number: | AB-80549 |
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-98 of human CXCL10/IP-10 |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:1000 - 1:5000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 11kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; CXCL10/IP-10 |
Applications
Western Blot: 1:1000 - 1:5000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-98 of human CXCL10/IP-10