CXCL12 / SDF-1 Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CXCL12 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | AB-84308 |
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SDF-1. AA range:44-93 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 ELISA:1/40000 |
| Molecular Weight: | 10kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | CXCL12; SDF1; SDF1A; SDF1B; Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF |
Applications
Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 ELISA:1/40000
Immunogen
The antiserum was produced against synthesized peptide derived from human SDF-1. AA range:44-93