DDDDK-Tag Rabbit Monoclonal Antibody
Western blot analysis of 293T, 293T transfected with GSK3B Protein , 293T transfected with BCAT2-N Protein and 293T transfected with BCAT2-C Protein, using DDDDK-Tag antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB-94792 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC5111-01 |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Reactivity: | Species independent |
| Applications: | Western Blot: 1:2000 - 1:10000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmistry: 1:50 - 1:200 Immunoprecipitation: 1:100 - 1:500 Flow Cytoemtry: 1:50 - 1:200 ChIP: 1:50 - 1:200 |
| Molecular Weight: | 56kDa/50kDa/46kDa/68kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | DDDDK;DDDDK tag;DDDDK-tag |
Applications
Western Blot: 1:2000 - 1:10000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmistry: 1:50 - 1:200 Immunoprecipitation: 1:100 - 1:500 Flow Cytoemtry: 1:50 - 1:200 ChIP: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to DDDDK tag.