Desmin

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus. Exposure time: 30s

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 30s

Catalog Number:AB-82627
Conjugate:Unù
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-470 of human Desmin.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:100
Molecular Weight:55kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:DES;CSM1;CSM2;LGMD2R;desmin;Desmin

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-470 of human Desmin.