Dopamine Receptor D2

Working Dilutions Primary Antibody:  1:300
Tests performed by Fusco F.R., Giampà C. Neuroanatomy Lab. Fondazione S. Lucia, Roma

Working Dilutions Primary Antibody: 1:300 Tests performed by Fusco F.R., Giampà C. Neuroanatomy Lab. Fondazione S. Lucia, Roma

Catalog Number:AB-11173
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Reactivity:Hu, Rt, Ms
Applications:Elisa, Western Blot, Immunocytochemistry, Immunohistochemistry (frozen tissues) Immunocytochemistry: 1:5000 dilution (PLP fixed rat brain sections) Western Blot: whole rat brain homogenate resulted in a pair of bands being detected at ~48 and ~51 kD at 1:1000-1:10000 with ECL Detection process. In some cases, it is required a higher dilution. For IHC (F) & ICC it is important to fix the cells or tissues with the PLP fixative (Please see protocol below)
Purification:Serum
Form:Liquid

Applications

Elisa, Western Blot, Immunocytochemistry, Immunohistochemistry (frozen tissues) Immunocytochemistry: 1:5000 dilution (PLP fixed rat brain sections) Western Blot: whole rat brain homogenate resulted in a pair of bands being detected at ~48 and ~51 kD at 1:1000-1:10000 with ECL Detection process. In some cases, it is required a higher dilution. For IHC (F) & ICC it is important to fix the cells or tissues with the PLP fixative (Please see protocol below)