Dopamine Receptor D2

Working Dilutions Primary Antibody: 1:300 Tests performed by Fusco F.R., Giampà C. Neuroanatomy Lab. Fondazione S. Lucia, Roma
| Catalog Number: | AB-11173 |
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Elisa, Western Blot, Immunocytochemistry, Immunohistochemistry (frozen tissues) Immunocytochemistry: 1:5000 dilution (PLP fixed rat brain sections) Western Blot: whole rat brain homogenate resulted in a pair of bands being detected at ~48 and ~51 kD at 1:1000-1:10000 with ECL Detection process. In some cases, it is required a higher dilution. For IHC (F) & ICC it is important to fix the cells or tissues with the PLP fixative (Please see protocol below) |
| Purification: | Serum |
| Form: | Liquid |
Applications
Elisa, Western Blot, Immunocytochemistry, Immunohistochemistry (frozen tissues) Immunocytochemistry: 1:5000 dilution (PLP fixed rat brain sections) Western Blot: whole rat brain homogenate resulted in a pair of bands being detected at ~48 and ~51 kD at 1:1000-1:10000 with ECL Detection process. In some cases, it is required a higher dilution. For IHC (F) & ICC it is important to fix the cells or tissues with the PLP fixative (Please see protocol below)
