EGFR / HER1 Rabbit Polyclonal Antibody
Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.
| Catalog Number: | AB-81864 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:1000 - 1:5000 mmunohistochemistry(paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence: 1:100 - 1:500 Immuncytochemistry: 1:100 - 1:500 IImmunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Molecular Weight: | 175kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; EGFR |
Applications
Western Blot: 1:1000 - 1:5000 mmunohistochemistry(paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence: 1:100 - 1:500 Immuncytochemistry: 1:100 - 1:500 IImmunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR