eNOS Rabbit Monoclonal Antibody

Western blot analysis of various lysates using eNOS Rabbit mAb  at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  
Exposure time: 180s.

Western blot analysis of various lysates using eNOS Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.

Catalog Number:MAB20985
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC51618
Immunogen:Recombinant protein.This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat
Applications:WB 1:1000 - 1:2000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:148kDa
Purification:Affinity purification
Form:liquid
Synonyms:eNOS; ECNOS

Applications

WB 1:1000 - 1:2000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant protein.This information is considered to be commercially sensitive.