Fibronectin
Western blot analysis of extracts of various cell lines, using Fibronectin antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.
| Catalog Number: | AB-84019 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin). |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 1:200 Immunofluorescence: 1:50 - 1:200 |
| Molecular Weight: | 290kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; fibronectin |
Applications
Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 1:200 Immunofluorescence: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin).