Flag-Tag Rabbit Monoclonal Antibody
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSK3B Protein,BCAT2-N Protein,BCAT2-C Protein, using DDDDK-Tag antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | AB092 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC5111-01 |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Species independent |
| Applications: | WB 1:5000 - 1:20000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF/ICC 1:300 - 1:2000 FC 1:50 - 1:200 ChIP 5 μg antibody for 10 μg-15 μg of Chromatin ChIP-seq 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 56kDa/50kDa/46kDa/68kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | DDDDK; DDDDK tag; DDDDK-tag; DDDDK-Tag |
Applications
WB 1:5000 - 1:20000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF/ICC 1:300 - 1:2000 FC 1:50 - 1:200 ChIP 5 μg antibody for 10 μg-15 μg of Chromatin ChIP-seq 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.