GAPDH Mouse Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:10,000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit 
Exposure time:60s

Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:10,000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time:60s

Catalog Number:MAB-10578
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG1
Clone:6C5
Immunogen:Recombinant protein of human GAPDH
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:5,000-1:20,000 Immunofluorescence 1:200 Immunocytochemistry: 1:100-500
Molecular Weight:36kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:5,000-1:20,000 Immunofluorescence 1:200 Immunocytochemistry: 1:100-500

Immunogen

Recombinant protein of human GAPDH