GFAP Mouse Monoclonal Antibody

This is one of a series of formalin fixed paraffin embedded horse brain samples .The brains from this series were removed whole, sliced incompletely cross ways and fixed over a 10-20 day period. The whole brain was put in a large necropsy specimen bucket containing 1-2 gallons of fixative, and after 24 hours, the formalin was poured off and then fresh formalin was added. The samples were fixed and embedded in paraffin in 2007 and sections were kept at room temperature until 2011. The sections were processed for antigen retrieval by boiling in pH=6 Citrate buffer for 10 min. Primary incubation with 5C10 was for 1 hour at 37°C, and secondary antibody incubation and color reaction was performed using the Vector mouse ABC kit.