Immunological Sciences

GFP Tag Mouse Monoclonal Antibody

Western blot analysis of over- expressed GFP fusion protein in 293 cell using GFP-Tag antibody ,at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti- Mouse IgG (H+L)) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s

Western blot analysis of over- expressed GFP fusion protein in 293 cell using GFP-Tag antibody ,at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti- Mouse IgG (H+L)) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s

Catalog Number:MAB-94345
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/mL
Host:Ms
Isotype:IgG
Clone:GFP28
Immunogen:Recombinant protein of GFP
Reactivity:All Species
Applications:• Western Blot: 1:2,500 • Immunofluorescence: 1:100 • Immunoprecipitation: 1:100
Molecular Weight:37kDa
Purification:Aff. Pur.
Form:Liquid

Applications

• Western Blot: 1:2,500 • Immunofluorescence: 1:100 • Immunoprecipitation: 1:100

Immunogen

Recombinant protein of GFP