GLI-3 Rabbit Monoclonal Antibody

Western blot analysis of extracts of HeLa cells, using Gli3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.
| Catalog Number: | MAB-94798 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC1941 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmisty: 1:50 - 1:200 |
| Molecular Weight: | 190 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; Gli3 |
Applications
Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmisty: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3
