GLI-3 Rabbit Monoclonal Antibody

Western blot analysis of extracts of HeLa cells, using Gli3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico  plus.
Exposure time: 180s.

Western blot analysis of extracts of HeLa cells, using Gli3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.

Catalog Number:MAB-94798
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC1941
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmisty: 1:50 - 1:200
Molecular Weight:190 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; Gli3

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytcoehmisty: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3