GRP-94

Western blot analysis of extracts of various cell lines, using GRP-94  antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using GRP-94 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-83479
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100
Molecular Weight:110kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:HSP90B1;ECGP;GP96;GRP94;HEL-S-125m;HEL35;TRA1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1.