HA Tag Mouse Monoclonal Antibody
Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) t 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | MAB-91878 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/mL |
| Host: | Ms |
| Isotype: | IgG1,Kappa |
| Clone: | 2D10 |
| Immunogen: | A synthetic peptide corresponding to HA tag. |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:2000 - 1:10000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | HA;HA tag;HA-tag |
Applications
Western Blot: 1:2000 - 1:10000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Immunogen
A synthetic peptide corresponding to HA tag.