HER1 / EGFR

Western blot analysis of extracts of OVCAR3 cells, using EGFR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

Western blot analysis of extracts of OVCAR3 cells, using EGFR Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:MAB-94446
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG2b
Clone:IS-23
Immunogen:A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50- 1:200 Immunofluorescence: 1:50-1:200 Immunoprecipitation: 1:50-1:200
Molecular Weight:175kDa
Purification:Affinity-chromatography
Form:Liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50- 1:200 Immunofluorescence: 1:50-1:200 Immunoprecipitation: 1:50-1:200

Immunogen

A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.