HER1 / EGFR

Western blot analysis of extracts of OVCAR3 cells, using EGFR Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB-94446 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG2b |
| Clone: | IS-23 |
| Immunogen: | A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50- 1:200 Immunofluorescence: 1:50-1:200 Immunoprecipitation: 1:50-1:200 |
| Molecular Weight: | 175kDa |
| Purification: | Affinity-chromatography |
| Form: | Liquid |
Applications
Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50- 1:200 Immunofluorescence: 1:50-1:200 Immunoprecipitation: 1:50-1:200
Immunogen
A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
