HER1 / EGFR
Western blot analysis of extracts of A-431 cells, using EGFR antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST
| Catalog Number: | AB-10103 |
| Size: | 100 ul |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Reactivity: | Ms, Rt, Hu, Ha, Ch |
| Applications: | Western blotting: 1:1000 The antigen should be separated in SDS-PAGE under non reducing condition. When using a secondary antibody labelled with peroxidase and ECL development. Immunoprecipitation: Optimal dilutions should be determined by end user. Elisa: Optimal dilutions should be determined by end user. |
| Molecular Weight: | 170kDa |
| Purification: | Serum |
| Form: | Liquid |
Applications
Western blotting: 1:1000 The antigen should be separated in SDS-PAGE under non reducing condition. When using a secondary antibody labelled with peroxidase and ECL development. Immunoprecipitation: Optimal dilutions should be determined by end user. Elisa: Optimal dilutions should be determined by end user.