HIF-1 alpha Rabbit Polyclonal Antibody

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA (F:GCCACAUUCACGUAUAUGATT,R:UCAUAUACGUGAAUGU GGCTT),(Green) primary antibody was diluted at 1:1000,4°over night, AF 800 secondary antibody.
| Catalog Number: | AB-84798 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Immunofluorescence: 1:50-200 Western Blot: 1/500 - 1/2000 Immunoprecipitation: 1:200 Immunohistochemistry: 1/100 - 1/300 |
| Molecular Weight: | 92-130kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; |
Applications
Immunofluorescence: 1:50-200 Western Blot: 1/500 - 1/2000 Immunoprecipitation: 1:200 Immunohistochemistry: 1/100 - 1/300
Immunogen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
