HIS Tag Mouse Monoclonal Antibody
Western blot analysis of various lysates, using His-Tag mAb at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.
| Catalog Number: | MAB-91879 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/mL |
| Host: | Mouse |
| Isotype: | IgG1 |
| Clone: | B8 |
| Immunogen: | A synthetic peptide corresponding to His tag. |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:5000-1:20000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 |
| Molecular Weight: | 27kDa/35kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | His;His tag;His-tag |
Applications
Western Blot: 1:5000-1:20000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to His tag.