Histone H3 Rabbit Polyclonal Antibody
Western blot analysis of various lysates using Histone H3 Rabbit pAb) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | ABE1054 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3. |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Applications: | <strong>Western Blot</strong>: 1:2000 - 1:10000 <strong>Immunohistochemistry (paraffin-embedded tissues):</strong> 1:50 - 1:200 <strong>Immunofluorescence</strong>:1:50 - 1:200 <strong>Immunocytochemistry:</strong> 1:50 - 1:200 <strong>Immunoprecipitation:</strong> 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells <strong>ELISA</strong> Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. <strong>ChIP</strong> 3μg antibody for 5μg-10μg of Chromatin |
| Molecular Weight: | 17kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3 |
Applications
<strong>Western Blot</strong>: 1:2000 - 1:10000 <strong>Immunohistochemistry (paraffin-embedded tissues):</strong> 1:50 - 1:200 <strong>Immunofluorescence</strong>:1:50 - 1:200 <strong>Immunocytochemistry:</strong> 1:50 - 1:200 <strong>Immunoprecipitation:</strong> 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells <strong>ELISA</strong> Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. <strong>ChIP</strong> 3μg antibody for 5μg-10μg of Chromatin
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3.