HMGB1
Western blot on different Cells : Positive samples 293T, MCF7, Jurkat, Mouse spleen, Mouse lung, Mouse kidney, Rat lung Western blot analysis of extracts of various cell lines, using HMGB1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit Exposure time: 60s.
| Catalog Number: | AB-83934 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Western Blot: 1:500 - 1: 2000 Immunohistochemistry: 1: 50 - 1: 200 Immunofluorescence: 1: 50 –1: 200 |
| Molecular Weight: | 25kDa |
| Purification: | Purified |
| Form: | Liquid |
| Synonyms: | HMGB1; HMG-1; HMG1; HMG3; SBP-1; high mobility group box 1 |
Applications
Western Blot: 1:500 - 1: 2000 Immunohistochemistry: 1: 50 - 1: 200 Immunofluorescence: 1: 50 –1: 200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1