HSP70 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 10s.
| Catalog Number: | AB-84218 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:1000 - 1:3000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | HSPA1A;HEL-S-103;HSP70-1;HSP70-1A;HSP70.1;HSP7 0I;HSP72;HSPA1;HSP70 |
Applications
Western Blot: 1:1000 - 1:3000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70.
