HSP70 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell  lines, using HSP70 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 10s.

Catalog Number:AB-84218
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:1000 - 1:3000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:70kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:HSPA1A;HEL-S-103;HSP70-1;HSP70-1A;HSP70.1;HSP7 0I;HSP72;HSPA1;HSP70

Applications

Western Blot: 1:1000 - 1:3000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70.